Scientific Program

Wednesday, February 25, 2009

 

POSTERS PRESENTATIONS

 

 

Poster Presentations will be installed in the Room TERRAZA. Posters should be up by 8:00 h and down by 22:00 h the same day. Authors should be at their posters the corresponding day from 17:30 to 19:30 h.

 

Session I A : Posters 1 to 6 (17:30 - 19:30)

 

Genetics and Cell Division

Chairpersons: Carol Gilchrist, Anuradha Lohia, Mario A. Rodríguez

 

In silico analysis of a novel heat shock transcription factors family in Entamoeba histolytica

Consuelo Gómez*, María de la Luz Macias, Olivia Medel, Cesar S. López, D. Guillermo Pérez, Blanca Buenrostro, Virginia Sánchez

 

Validation and pertinence of microarrays representing all the predicted genes of Entamoeba histolytica

Christian Weber*, Julien Santi-Rocca, Nancy Guillén

 

Cloning and biochemistry characterization of DNA polymerase from Entamoeba histolytica

Pastor Palacios G.*, Azuara Liceaga E., Brieba, LG.

 

Relation of the EhPGP5 inhibition with programmed cell death in Entamoeba histolytica

María Olivia Medel.*, Consuelo Gómez., Virginia Sánchez, Mineko Shibayama., José D'Artagnan Villalba., Guillermo Pérez, Ishiwara.

 

Genetic heterogeneity results from intercellular variation in DNA content in Entamoeba histolytica

Chandrama Mukherjee, Shubhra Majumder, Anuradha Lohia*

 

Transcriptional profiling of the amoebapore-silenced G3 trophozoites: analysis of a significantly up-regulated gene.

Rivka Bracha*, Yael Nuchamowitz, Carol A. Gilchrist, William A. Petri Jr, David Mirelman

 

Discussion (19:00 - 19:30)

 

 

Session I B : Posters 7 to 12 (17:30 - 19:30)

 

Gene Expression

Chairpersons: Consuelo Gómez, Nancy Guillén, Upinder Singh, César López-Camarillo

Entamoeba histolytica has open reading frames that suppress the peptidyl-tRNA hydrolase Ts mutation in Escherichia coli

Gloria Leon-Avila*, José Manuel Hernández, Gabriel Guarneros, Minerva Camacho, Juan Pedro Luna, Armando Pérez, Alma Jimenez, Carolina Maza, Isabel Salazar, Benjamin Nogueda

 

Diversity analysis of short tandem repeats region from the chitinase gene in Entamoeba species

Gabriela García*, Rodrigo Gutiérrez, Alicia Valadez, Fernando Ramos, Patricia Morán, Enrique González, Jorge Yáñez, Paul Gaytán, Cecilia Ximénez

 

DNA ligase I from Entamoeba histolyica

César S. Cardona Fé1ix*, Guillermo Pastor Palacios, Luis G. Brieba

 

Effect of the flavonoid (-)-epicatechin in Entamoeba histolytica morphology and gene expression

Jacqueline Soto, D. Guillermo Pérez, Consuelo Gómez, Virginia Sánchez, Fernando Calzada, Ma. Esther Ramírez*

 

Differential gene expression during programmed cell death in Entamoeba histolytica: identification of apoptotic cell signals

Virginia Sánchez Monroy*, Ma. Olivia Medel Flores, José DŪArtagnan Villalba-Magdaleno, Consuelo Gómez, David Guillermo Pérez Ishiwara

 

In silico analysis and detection of mRNA transcripts for the ESCRT machinery in Entamoeba histolytica

Israel López-Reyes*, Guillermina García-Rivera, Cecilia Bañuelos- Arturo Barrón González-Robles, César López-Camarillo, Laurence Marchat, Esther Orozco

 

Discussion (19:00 - 19:30)

 

 

Session I C : Posters 13 to 26(17:30 - 19:30)

 

Protein Expression

Chairpersons: Elisa Azuara, Luis Brieba, Tomoyoshi Nozaki, Ibne Karin M. Ali, Serge Ankri

 

Mono-ADP-ribosyl transferase activity and the possible genes codifying for the enzyme in Entamoeba histolytica

Rafael Juárez, Ángeles Cervantes, Catalina A. Fuentes, Ángel H. Álvarez, Iris Hernández, Mónica Silva, Guadalupe Martínez-Cadena, Eva E. Avila*

Over-expression of a mutated EhRabB protein decreases the phagocytosis of Entamoeba histolytica trophozoites

Rosa María García-Pérez*, León J. Juárez-Hernández, Guillermina García-Rivera, Esther Orozco, Mario A. Rodríguez

 

Identification and characterization of two MYB transcription factors in Entamoeba histolytica

Eric Meneses*, Helios Cárdenas, Sandra Morales, Esther Orozco, Luis Brieba, Elisa Azuara

 

Preliminary characterization of a truncated version of the TATA binding protein from Entamoeba histolytica

Sandra Morales-Arrieta*, Elisa Azuara-Liceaga, Rocío Rodríguez-Rocha, Luis G. Brieba

 

Identification of an Entamoeba histolytica sequence-specific URE1-binding protein with homology to mammalian p100

Mercedes Calixto-Gálvez*, Alejandro García-Muñoz, Mónica Romero-Díaz, Israel López-Reyes, Mario A. Rodríguez

 

Identification of a small family of poly(A) ribonucleases in Entamoeba histolytica and characterization of EhCAF1 deadenylase

Itzel López-Rosas*, Laurence A. Marchat, Esther Orozco, Olga N. Hernández de la Cruz, César López-Camarillo

 

Expression of EhMyb SHAQKYF genes on Entamoeba histolytica

Helios Cárdenas*, Selene Zárate, Esther Orozco, Luis Brieba, Minerva Camacho, Elisa Azuara

 

Cloning and expression of the EhBLM DNA-helicase in Entamoeba histolytica

Ma. del Socorro Charcas-López*, Cesar López-Camarillo, Mavil López-Casamichana, Israel López Reyes, Esther Orozco, Absalom Zamorano, Laurence A. Marchat

 

Genome-wide analysis identified novel genes involved in DNA damage response induced by UV-C irradiation in Entamoeba histolytica

Christian Weber, Laurence A. Marchat, Nancy Guillen, César López- Camarillo*.

 

Structural characteristics of EhCP112 cysteine proteinase sequence

Rodrigo Arreola*, Luis G. Brieba, Jaime Ortega-López

 

Biochemical characterization of recombinant cysteine proteinase Ehcp112 from Entamoeba histolytica

Laura Itzel Quintas Granados*, Esther Orozco, Luis G. Brieba, Rossana Arroyo Verastegui, Jaime Ortega López

 

Characterization of the putative polyadenylation and transcriptional factor EhPC4 of Entamoeba histolytica

Olga N. Hernández de la Cruz*, Laurence A. Marchat, Itzel López-Rosas, Esther Orozco, César López Camarillo

 

Identification of Entamoeba histolytica proteins interacting with the carboxy-terminus of the›› EhADH112

Silvia Castellanos Castro*, Máximo Martínez Benitez, Esther Orozco

 

Molecular basis of unusual catalytic preference to GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase

Rusely Encalada*, Arturo Rojo-Domínguez, José Salud Rodríguez Zavala, Juan Pablo Pardo, Héctor Quezada, Rafael Moreno-Sánchez, Emma Saavedra

 

Discussion (19:00 - 19:30)

 

 

Scientific Program

Thursday, February 26, 2009

 

Session II A : Posters 1 to 9 (17:30 - 19:30)

 

Cell Signaling and Cytoskeleton

Chairpersons: Patricia Talamás, Lesly Temesvari, Sarah Buss, Alok Bhattacharya

 

Studies into the interactions of transmembrane kinases of Entamoeba histolytica with extracellular ligands during amoebic phagocytosis and ingestion

Nathaniel Christy*, Sarah Buss, William A. Petri Jr.

 

Kinetics of intracellular traffic of the Entamoeba histolytica integrin-like molecule (β1EhFNR)

Silvana Pertuz-Belloso*, Verónica Ivonne Hernández-Ramírez, Amelia Angel-Martínez, Victor H. Rosales, Patricia Talamás-Rohana

 

Functional study and subcellular organization of the EhABP152 protein from Entamoeba histolytica

Alma Delia Campos-Parra*, María de Jesús Almaraz Barrera, Miguel Angel Vargas Mejía

 

Entamoeba histolytica: characterization of vesicles with fibrilar content present in trophozoites recovered from hepatic liver abscesses from hamster

Norma Cristina Segovia-Gamboa*, Yolanda Medina-Flores, Verónica Ivonne Hernández-Ramírez, Amelia Angel-Martínez, Viola Leticia Pérez-Castillo, Patricia Talamás-Rohana, Bibiana Chávez-Munguía

 

EhADH is a Brol-like domain containing protein involved in the Entamoeba histolytica multivesicular bodies endocytic pathway

Cecilia Bañuelos*, Guillermina García-Rivera, Leobardo Mendoza, Arturo González Robles, Silvia Herranz, Oliver Vincent, Israel López-Reyes, Esther Orozco

 

Detection of Entamoeba histolytica Calreticulin in tissue lesions of amebic liver abscess

Enrique González*, Angélica Limón, Ma. Del Carmen García de León, Alfonso Olivos, Mario Nequiz, Gabriela García, Fernando Ramos, Patricia Morán, Alicia Valadez, Liliana Rojas, Cecilia Ximénez

 

A novel Entamoeba histolytica myosin II heavy chain is expressed in trophozoites and is part of functional myosin II complexes

J. Daniel Díaz-Valencia*, N. Villegas-Sepúlveda, Isaura Meza

 

Phosphatidylinositol (3,4,5)-trisphosphate is important in the extension of pseudopodia and closure of erythrophagosomes in Entamoeba histolytica

Lesly A. Temesvari*, Yevgeniya A. Byekova, Rhonda R. Powell

 

Vesicular traffic of the fibronectin receptor (β1 EhFNR ) in trophozoites stimulated with fibronectin (FN) and trophozoite recovered from liver abscesses

Rosario Javier-Reyna*, Verónica Ivonne Hernández-Ramírez, Carlos Osorio, Donaciano Flores-Robles, José Luis Rosales-Encina, Amelia Angel-Martínez, Blanca Reyes-Márquez, Patricia Talamás-Rohana.

Discussion (19:00 - 19:30)

 

 

Session II B : Posters 10 to 18 (17:30 - 19:30)

 

Biochemistry: Metabolism, Proteases

Chairpersons: Fernando Anaya, Sharon Reed, James McKerrow, Felipe Padilla

 

An inverzincin from Entamoeba histolytica

Minerva Barrios-Ceballos*, Felipe Padilla-Vaca, David Mirelman

 

Effect of protease inhibitors and chloroquine on digestion of erythrocytes in Entamoeba histolytica

Fernando Anaya-Velázquez*, Martha Laura Caracheo-Delgado, Felipe Padilla-Vaca, María Luisa Lazo de la Vega-Monroy

 

Purification of a proteinic complex with protease activity from Entamoeba histolytica HM1:IMSS

A.González-Canto*, R. López Vancell, N. Salaiza Suazo, R. Arreguín Espinosa, M. Néquiz Avendaño, O. García Hernández, R. Pérez Tamayo

 

Pyruvate: ferredoxin oxidoreductase is essential for energy metabolism under oxidative stress in Entamoeba histolytica

Erika Pineda *, Alfonso Olivos, Emma Saavedra

 

Biochemical changes induced by cholesterol liposomes in Entamoeba histolytica.

José de Jesús Serrano-Luna*, Juan Manuel Gutiérrez-Meza, Ricardo Mejía-Zepeda,Víctor Tsutsumi, Mineko Shibayama

 

Efficient excystation of in vitro generated cysts of Entamoeba invadens

Biswa Nath Mitra*, Gabriele Pradel, Daniel Eichinger

 

EhHMBP75 protein is expressed under iron starvation conditions and is involved in iron acquisition from Hb in Entamoeba histolytica

A Cruz-Castañeda*, J. Hernández-Sanchez, M. López-Casamichana, S. Zárate, E. Azuara-Liceaga, J.J. Olivares-Trejo

 

EhHMBP26 and EhHMBP45 from Entamoeba histolytica bind hemoglobin in acidic pH

A. Cruz-Castañeda*, J. Hernández-Sánchez, M. López-Casamichana Selene, E. Zárate, J. Azuara-Liceaga., J. Olivares-Trejo JJ.

 

Modulation of a cyclooxygenase (COX)-like enzyme in entamoeba histolytica during the adhesion process

Luilli López*, Donaciano Flores-Robles, Verónica Ivonne Hernández-Ramírez, José Alfredo Díaz-Gandarilla, Amelia Angel, Patricia Talamás-Rohana

 

Discussion (19:00 - 19:30)

 

 

Session II C : Posters 19 to 24(17:30 - 19:30)

 

Host Interactions

Chairpersons: David Mirelman, Ruy Pérez-Tamayo, Mineko Shibayama, Isaura Meza

 

Electron dense granules of Entamoeba histolytica trophozoites recovered from liver lesions: interaction with MDCK cell monolayers

Bibiana Chávez-Munguía*, Guadalupe Castañon-Gutiérrez, Amelia-Angel Martínez, Patricia Talamás-Rohana, Adolfo Martínez-Palomo

 

Entamoeba histolytica: oxygen resistance and virulence

Espiridión Ramos-Martínez*, Alfonso Olivos-García, Emma Saavedra, Mario Nequiz, Cuauhtémoc E. Sánchez, Eusebio Tello, Mohamed El-Hafidi, Andrés Saralegui, Erika Pineda, José S Delgado, Irmgard Montfort, Ruy Pérez-Tamayo

 

New insights into amoeba-bacteria relationship

Felipe Padilla-Vaca*, Claudia Mendoza-Macías, Minerva Barrios-Ceballos, Ángeles Rangel-Serrano, Fernando Anaya-Velázquez, Rivka Bracha, David Mirelman

 

Expression and localization of gal/galnac in the virulent and non virulent variants from E. histolytica HM1:IMSS

Rosario López Vancell*, Roberto Arreguín Espinosa, Mario Néquiz Avendaño, Augusto González Canto, Dora López Vancell, Ruy Pérez Tamayo

 

Study of the encystment process in Entamoeba invadens

Norma Cristina Segovia-Gamboa*, Yolanda Medina-Flores, Verónica Ivonne Hernández-Ramírez, Bibiana Chávez-Munguía, Patricia Talamás-Rohana

 

The lysine and glutamic acid-rich protein 1 (KERP1) is involved in the early steps of amoebic liver abscess development

Julien Santi-Rocca*, Nancy Guillén

 

Discussion (19:00 - 19:30)

 

 

Scientific Program

Friday, February 27, 2009

 

Session III A : Posters 1 to 7 (17:30 - 19:30)

 

Immunology: Vaccines, Cytokine Production

Chairpersons: Adolfo Martínez-Palomo, Kris Chadee, Blanca Sánchez, Jonathan Ravdin

 

Evaluation of immunogenicity and protective efficacy OFA pcDNA-EhCPADH vaccine in hamster

Máximo B. Martínez*, Mario A. Rodríguez, Guillermina García-Rivera, Tomás Sánchez, Rogelio Hernández-Pando, Diana Aguilar, Esther Orozco

 

Construction and evaluation of two T polyepitopes from the EhCPADH complex of Entamoeba histolytica in hamsters

Jonnatan Pais*, Máximo B. Martínez, Esther Orozco

 

Protein tyrosine phosphatase from Entamoeba histolytica modulates the secretion of cytokines and translocation of nuclear factor NFkB from macrophages

Juan Espejel-Zaragoza, Alma Escalona-Montaño, Mario Nequiz, Amelia Angel, Laila Gutiérrez-Kobeh, Ruy Pérez-Tamayo, Patricia Talamás-Rohana, María Magdalena Aguirre-García*

 

Cytokine production in leukocytes of patients from ALA exposed to the monocyte locomotion inhibitory factor produced by Entamoeba histolytica

Sara Rojas-Dotor*, Patricia Morán Silva, Cecilia Ximénez García, Guadalupe Rico Rosillo

 

Expression of immune modulator cytokines in human fulminant amebic colitis

Javier Ventura-Juárez*, RE. Sierra-Puente, R. Campos-Rodríguez, RA. Jarillo-Luna, L. Muñoz-Fernández

 

Expression of prostaglandin EP2 and EP4 receptors during cyclooxygenase-2 induction in macrophages stimulated with Entamoeba histolytica antigens

Hiriz Chaparro-Reyes*, Gabriela Sosa-Robledo, Carmen González Horta, Gilberto Erosa De La Vega, Patricia Talamás Rohana, Blanca Sánchez Ramírez

 

Expression of prostaglandin EP2 and EP4 receptors during amoebic liver abscess formation in hamsters

Celia Quiñones Flores*, Ana Rosa Hernández Garibay, Ernesto Ramos Martínez, Carmen González Horta, Gilberto Erosa De La Vega, Porfirio Gutiérrez Martínez, Patricia Talamás Rohana, Blanca Sánchez Ramírez

 

Discussion (19:00 - 19:30)

 

 

Session III B : Posters 8 to 13(17:30 - 19:30)

 

Experimental Amebiasis

Chairpersons: Sarah A Evans, Mineko Shibayama, Easwaran Variyam

Kinetics of cytokines production in the amoebic liver abscess of hamster

José Manuel Galván Moroyoqui*, Judith Pacheco-Yépez, Víctor Tsutsumi, Isaura Meza, Mineko Shibayama

 

Entamoeba histolytica induces apoptosis in both inflammatory cells and hepatocytes using hamster model

José D•Artagnan Villalba-Magdaleno*, Silvia Galindo Gómez, Guillermo Pérez Ishiwara, Mineko Shibayama

 

A Brazilian species of Entamoeba dispar produces amoebic liver abscess with an important protease activity

Silvio Dolabella*, Edward Silva, Jesús Serrano-Luna, Víctor Tsutsumi, Mineko Shibayama

 

Morphometric changes in Entamoeba histolytica trophozoites present in liver abscess in misoprostol- treated BALB/c mice

Blanca Sánchez-Ramírez*, Andrés Aceves-Cano, Carmen González-Horta, Margarita Levario-Carrillo, Blanca Rivera-Chavira, Rocío Infante-Ramírez, Patricia Talamás-Rohana

 

Prolonged noninvasive intestinal amebiasis in gnotobiotic rat

Easwaran P. Variyam*, Gordon Brackee

 

Involvement of IL-4, IFN1g, TNF-a and cyclophilin in the development of the amoebic liver abscess

María del Pilar Crisóstomo-Vázquez *, Leticia Eligio García., Victor A. Maravélez Acosta, Jorge Hernández-García, Andrés Flores-Luna, Enedina Jiménez-Cardoso.

 

Discussion (19:00 - 19:30)

 

 

Session III C :Posters 14 to 20(17:30 - 19:30)

 

Therapeutics

Chairpersons: Michael Duchêne, Maria Porfiria Barrón, Iris Bruchhaus

 

In vitro antiamoebic activities of fractions from a medical plant from Cameroun

Flore Melanie *, Hubert Donfack, Emmanuel Njoya, Moundipa Paul Bilong-Bilong, Egbert Tannich, Iris Bruchhaus

 

In vitro and toxicity studies of Codiaeum variegatum on Entamoeba histolytica.

Gondame Flore Mélanie *, Paul Moundipa Fewou, Bilong-Bilong, CF, I. Bruchhaus

 

Extracts from marine actinomycetes as novel anti amebic agents

Erica Ryke, Aaron Socha, David Rowley, Avelina Espinosa*

 

Antiamoebic effect of methanolic extracts from four species of family cactacea

Eufemia Morales R.*, Jaime Treviño N., Ruth Garza P., Mario Morales V., Azucena Oranday C., María Porfiria Barrón G.

 

Determination of the inhibitory concentration 50% (IC50) of methanolic extracts of Ariocarpus kotschoubeyanus, Ariocarpus retusus and Fouquieria splendens on axenic growth of Entamoeba histolytica

Ramón Gerardo Rodríguez Garza*, María Eufemia Morales Rubio, María Julia Verde Star, Ruth Amelia Garza Padrón, Mario R. Morales Vallarta, María Porfiria Barrón González

 

Amoebicid activity evaluation of extract astrophytum myriostigma stem and in vitro callus

Ruth Amelia Garza Padrón*, Ma. Porfiria Barrón González, Ma. Julia Verde Star, Ma. Eufemia Morales Rubio, Ramón Gerardo Rodríguez Garza, Mario Morales Vallarta

 

Phagocytosis assays with Entamoeba histolytica as a model system to define biocompatibility of multiwalled carbon nanotubes

Julio C. Carrero*, Ana L. Elías, Humberto Terrones, Morinobu Endo, Mauricio Terrones, Juan P. Laclette

 

Discussion (19:00 - 19:30)

 

 

Session III D: Posters 21 to 27(17:30 - 19:30)

 

Epidemiology and Diagnostic Tools

Chairpersons: Ji Dar-Der, William Petri Jr.,Christen Stensvold, Cecilia Ximénez

 

Infection frequency of Entamoeba species in a turtle collection in Mexico City

Rodrigo Gutiérrez*, Gabriela García, Alicia Valadéz, Fernando Ramos, Monica Salmerón, Patricia Morán, Enrique González, Jorge Yañez, Paul Gaytán, Cecilia Ximénez

 

Differentiation between Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using pyrosequencing and prevalence analysis of Entamoeba moshkovskii in patients positive for the Entamoeba complex diagnosed in Northwestern Europe

Christen R. Stensvold*, Marianne Lebbad, Jaco J. Verweij, Venkata R. S. Geedi, JĮrgen S. Jensen, Cathrine Jespersgaard, Georg von Samson-Himmelstjerna, Henrik V. Nielsen

 

Isolation and identification of Acanthamoeba species related to amebic encephalitis and non-pathogenic Vannella species in Taiwan

Liang Shih-Yu *, Ji Dar-Ren, Hsia Kan-Tai, Hsu Bing-Mu, Chen Jung-Sheng, Wu Ming-Huei, Lai Cheng-Hao, Ji Dar-Der

 

The heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity

Kyoung-Ju Song*

 

Immunodominant low molecular weight recombinant protein of Entamoeba histolytica expressed by Escherichia. coli

Dinora Pérez*, R. Rangel, A. Rojo, G. Mendoza, B. González, D. Barrientos J. Viader, E. Tamez, R. Tijerina, F. Bosques, A. Isibasi, L. Galán, K. Arévalo, MS. Flores

 

PCR amplification of different DNA regions of Entamoeba histolytica species performed in amebic or pyogenic liver abscesess in Mexico

Liliana Rojas*, Linda García, Olivia Valenzuela, Fernando Ramos, Patricia Morán, Gabriela García, Alicia Valadez, Enrique González, Angélica Limón, Erick Hernandez, Cecilia Ximénez

 

Development of loop-mediated isothermal amplification assay for diagnosis of Entamoeba histolytica

Ji Dar-Der *, Liang Shih-Yu, Chan Yun-Hsien, Hsia Kan-Tai, Lee Jing-Lun, Kuo Ming-Chu, Hwa Kuo-Yuan, Chan Chi-Wen, Chiang Ting-Yi, Chen Jung-Sheng, Wu Fang-Tzy

 

Discussion (19:00 - 19:30)