Scientific Program
POSTERS PRESENTATIONS
Poster Presentations will be installed in the Room TERRAZA. Posters should be up by 8:00 h and down by 22:00 h the same day. Authors should be at their posters the corresponding day from 17:30 to 19:30 h.
Session I A : Posters 1 to 6 (17:30 - 19:30)
Genetics and Cell Division
Chairpersons: Carol
Gilchrist, Anuradha Lohia, Mario A. Rodríguez
In silico
analysis of a novel heat shock transcription factors family in Entamoeba histolytica
Consuelo Gómez*,
María de la Luz Macias, Olivia Medel, Cesar S. López, D. Guillermo Pérez,
Blanca Buenrostro, Virginia Sánchez
Validation and pertinence of
microarrays representing all the predicted genes of Entamoeba histolytica
Christian Weber*, Julien
Santi-Rocca, Nancy Guillén
Cloning and biochemistry
characterization of DNA polymerase from Entamoeba histolytica
Pastor Palacios G.*, Azuara Liceaga E.,
Brieba, LG.
Relation of the EhPGP5
inhibition with programmed cell death in Entamoeba histolytica
María Olivia Medel.*, Consuelo
Gómez., Virginia Sánchez, Mineko Shibayama., José D'Artagnan
Villalba., Guillermo Pérez, Ishiwara.
Genetic heterogeneity results from intercellular variation in DNA
content in Entamoeba histolytica
Chandrama Mukherjee, Shubhra Majumder, Anuradha Lohia*
Transcriptional profiling of the amoebapore-silenced
G3 trophozoites: analysis of a significantly up-regulated
gene.
Rivka Bracha*, Yael Nuchamowitz,
Carol A. Gilchrist, William A. Petri Jr,
David Mirelman
Discussion
(19:00 - 19:30)
Session
I B : Posters 7 to 12 (17:30 - 19:30)
Gene
Expression
Chairpersons: Consuelo Gómez, Nancy Guillén, Upinder Singh, César
López-Camarillo
Entamoeba histolytica has open reading frames that suppress the peptidyl-tRNA hydrolase
Ts mutation in Escherichia coli
Gloria Leon-Avila*, José Manuel Hernández, Gabriel Guarneros, Minerva Camacho, Juan Pedro Luna, Armando Pérez, Alma Jimenez, Carolina Maza, Isabel Salazar, Benjamin Nogueda
Diversity analysis of short tandem
repeats region from the chitinase gene in Entamoeba
species
Gabriela
García*, Rodrigo Gutiérrez, Alicia Valadez, Fernando Ramos, Patricia Morán,
Enrique González, Jorge Yáñez, Paul Gaytán, Cecilia Ximénez
DNA ligase I
from Entamoeba
histolyica
César S. Cardona Fé1ix*, Guillermo Pastor Palacios, Luis G. Brieba
Effect of the flavonoid
(-)-epicatechin in Entamoeba histolytica morphology
and gene expression
Jacqueline Soto, D. Guillermo Pérez, Consuelo Gómez, Virginia Sánchez,
Fernando Calzada, Ma. Esther Ramírez*
Differential gene expression during
programmed cell death in Entamoeba
histolytica: identification of apoptotic cell signals
Virginia Sánchez Monroy*, Ma. Olivia Medel Flores, José DíArtagnan Villalba-Magdaleno, Consuelo Gómez, David Guillermo Pérez Ishiwara
In
silico analysis and detection of mRNA transcripts
for the ESCRT machinery in Entamoeba histolytica
Israel López-Reyes*,
Guillermina García-Rivera, Cecilia Bañuelos- Arturo Barrón González-Robles,
César López-Camarillo, Laurence Marchat, Esther Orozco
Discussion
(19:00 - 19:30)
Session I C : Posters 13
to 26Ý (17:30 - 19:30)
Protein Expression
Chairpersons: Elisa Azuara, Luis Brieba, Tomoyoshi
Nozaki, Ibne Karin M. Ali, Serge Ankri
Mono-ADP-ribosyl
transferase activity and the possible genes codifying for the enzyme in Entamoeba
histolytica
Rafael Juárez, Ángeles Cervantes, Catalina A.
Fuentes, Ángel H. Álvarez, Iris Hernández, Mónica Silva, Guadalupe
Martínez-Cadena, Eva E. Avila*
Over-expression of a mutated EhRabB protein decreases the phagocytosis of Entamoeba histolytica trophozoites
Rosa María
García-Pérez*, León J. Juárez-Hernández, Guillermina García-Rivera, Esther
Orozco, Mario A. Rodríguez
Identification
and characterization of two MYB transcription factors in Entamoeba
histolytica
Eric Meneses*, Helios Cárdenas, Sandra
Morales, Esther Orozco, Luis Brieba, Elisa Azuara
Preliminary characterization of a truncated version of the TATA binding protein from Entamoeba histolytica
Sandra
Morales-Arrieta*, Elisa Azuara-Liceaga, Rocío Rodríguez-Rocha, Luis G. Brieba
Identification of an Entamoeba histolytica sequence-specific URE1-binding protein with homology to mammalian p100
Mercedes Calixto-Gálvez*, Alejandro García-Muñoz, Mónica Romero-Díaz, Israel López-Reyes, Mario A. Rodríguez
Identification of a small family of poly(A) ribonucleases in Entamoeba histolytica and characterization of EhCAF1 deadenylase
Itzel López-Rosas*, Laurence A. Marchat,
Esther Orozco, Olga N. Hernández de la Cruz, César López-Camarillo
Expression of EhMyb SHAQKYF genes
on Entamoeba histolytica
Helios
Cárdenas*, Selene Zárate, Esther Orozco, Luis Brieba, Minerva Camacho, Elisa
Azuara
Cloning and expression of the EhBLM DNA-helicase in Entamoeba histolytica
Ma. del Socorro
Charcas-López*, Cesar López-Camarillo, Mavil López-Casamichana, Israel López
Reyes, Esther Orozco, Absalom Zamorano, Laurence A. Marchat
Genome-wide
analysis identified novel genes involved in
Christian Weber, Laurence A. Marchat, Nancy Guillen, César López- Camarillo*.
Structural characteristics of EhCP112 cysteine proteinase sequence
Rodrigo Arreola*, Luis G. Brieba,
Jaime Ortega-López
Biochemical characterization of recombinant cysteine proteinase Ehcp112 from Entamoeba histolytica
Laura Itzel Quintas Granados*, Esther Orozco, Luis G. Brieba, Rossana Arroyo Verastegui, Jaime Ortega López
Characterization of the putative
polyadenylation and transcriptional factor EhPC4
of Entamoeba histolytica
Olga N. Hernández
de la Cruz*, Laurence A. Marchat, Itzel López-Rosas, Esther Orozco, César López
Camarillo
Identification of Entamoeba histolytica proteins interacting with the
carboxy-terminus of theÝÝ EhADH112
Silvia Castellanos Castro*, Máximo Martínez Benitez, Esther Orozco
Molecular basis of unusual catalytic preference to GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase
Rusely Encalada*,
Arturo Rojo-Domínguez, José Salud Rodríguez Zavala, Juan Pablo Pardo, Héctor
Quezada, Rafael Moreno-Sánchez, Emma Saavedra
Discussion (19:00 - 19:30)
Scientific Program
Session II A : Posters 1
to 9 (17:30 - 19:30)
Cell Signaling and
Cytoskeleton
Chairpersons: Patricia
Talamás, Lesly Temesvari, Sarah Buss, Alok Bhattacharya
Studies into the interactions of transmembrane kinases of Entamoeba histolytica with extracellular ligands during amoebic phagocytosis and ingestion
Nathaniel Christy*, Sarah Buss, William A. Petri Jr.
Kinetics of intracellular traffic of the Entamoeba histolytica integrin-like molecule (β1EhFNR)
Silvana Pertuz-Belloso*, Verónica Ivonne Hernández-Ramírez, Amelia Angel-Martínez, Victor H. Rosales, Patricia Talamás-Rohana
Functional study and subcellular
organization of the EhABP152 protein
from Entamoeba histolytica
Alma Delia Campos-Parra*, María de Jesús Almaraz Barrera, Miguel
Angel Vargas Mejía
Entamoeba
histolytica: characterization of vesicles with fibrilar content present in
trophozoites recovered from hepatic liver abscesses from hamster
Norma Cristina Segovia-Gamboa*, Yolanda
Medina-Flores, Verónica Ivonne Hernández-Ramírez, Amelia Angel-Martínez, Viola
Leticia Pérez-Castillo, Patricia Talamás-Rohana, Bibiana Chávez-Munguía
EhADH is a Brol-like domain containing protein involved in the Entamoeba
histolytica multivesicular bodies endocytic pathway
Cecilia
Bañuelos*, Guillermina García-Rivera, Leobardo Mendoza,
Arturo González Robles, Silvia Herranz, Oliver
Vincent, Israel López-Reyes, Esther Orozco
Detection of Entamoeba histolytica Calreticulin in tissue lesions of amebic liver abscess
Enrique
González*, Angélica Limón, Ma. Del Carmen García de León, Alfonso Olivos, Mario
Nequiz, Gabriela García, Fernando Ramos, Patricia Morán, Alicia Valadez,
Liliana Rojas, Cecilia Ximénez
A novel Entamoeba histolytica myosin II heavy chain is expressed in trophozoites and is part of functional myosin II complexes
J. Daniel Díaz-Valencia*, N. Villegas-Sepúlveda, Isaura Meza
Phosphatidylinositol (3,4,5)-trisphosphate is important in the extension of pseudopodia and closure of erythrophagosomes in Entamoeba histolytica
Lesly A. Temesvari*, Yevgeniya A. Byekova, Rhonda R. Powell
Vesicular traffic of the fibronectin receptor (β1 EhFNR ) in trophozoites stimulated with fibronectin (FN) and trophozoite recovered from liver abscesses
Rosario
Javier-Reyna*, Verónica Ivonne Hernández-Ramírez, Carlos Osorio, Donaciano
Flores-Robles, José Luis Rosales-Encina, Amelia Angel-Martínez, Blanca
Reyes-Márquez, Patricia Talamás-Rohana.
Discussion
(19:00 - 19:30)
Session
II B : Posters 10 to 18 (17:30 -
19:30)
Biochemistry: Metabolism,
Proteases
Chairpersons: Fernando
Anaya, Sharon Reed, James McKerrow, Felipe Padilla
An
inverzincin from Entamoeba histolytica
Minerva Barrios-Ceballos*, Felipe Padilla-Vaca,
David Mirelman
Effect of protease inhibitors and
chloroquine on digestion of erythrocytes in Entamoeba histolytica
Fernando Anaya-Velázquez*, Martha Laura
Caracheo-Delgado, Felipe Padilla-Vaca, María Luisa Lazo de
Purification of a proteinic complex with protease activity from Entamoeba histolytica HM1:IMSS
A.González-Canto*, R. López Vancell, N. Salaiza Suazo, R. Arreguín Espinosa, M. Néquiz Avendaño, O. García Hernández, R. Pérez Tamayo
Pyruvate:
ferredoxin oxidoreductase is essential for energy metabolism under oxidative stress in Entamoeba histolytica
Erika Pineda *, Alfonso
Olivos, Emma Saavedra
Biochemical changes induced by cholesterol liposomes in Entamoeba histolytica.
José de Jesús Serrano-Luna*, Juan Manuel Gutiérrez-Meza, Ricardo
Mejía-Zepeda,Víctor Tsutsumi, Mineko Shibayama
Efficient excystation of in vitro generated cysts of Entamoeba invadens
Biswa Nath Mitra*, Gabriele
Pradel, Daniel Eichinger
EhHMBP75 protein is expressed under iron
starvation conditions and is involved in iron acquisition from Hb in Entamoeba
histolytica
A Cruz-Castañeda*, J. Hernández-Sanchez, M.
López-Casamichana, S. Zárate, E. Azuara-Liceaga, J.J. Olivares-Trejo
EhHMBP26 and EhHMBP45 from Entamoeba histolytica bind hemoglobin in acidic pH
A. Cruz-Castañeda*, J. Hernández-Sánchez, M. López-Casamichana Selene, E. Zárate, J. Azuara-Liceaga., J. Olivares-Trejo JJ.
Modulation of a cyclooxygenase (
Luilli López*,
Donaciano Flores-Robles, Verónica Ivonne Hernández-Ramírez, José Alfredo
Díaz-Gandarilla, Amelia Angel, Patricia Talamás-Rohana
Discussion
(19:00 - 19:30)
Session II C : Posters 19
to 24Ý (17:30 - 19:30)
Host Interactions
Chairpersons: David
Mirelman, Ruy Pérez-Tamayo, Mineko Shibayama, Isaura Meza
Electron dense granules of Entamoeba histolytica trophozoites recovered from liver
lesions: interaction with MDCK cell monolayers
Bibiana Chávez-Munguía*, Guadalupe Castañon-Gutiérrez, Amelia-Angel Martínez, Patricia Talamás-Rohana, Adolfo Martínez-Palomo
Entamoeba histolytica: oxygen resistance and virulence
Espiridión Ramos-Martínez*, Alfonso Olivos-García, Emma Saavedra,
Mario Nequiz, Cuauhtémoc E. Sánchez, Eusebio Tello, Mohamed El-Hafidi, Andrés
Saralegui, Erika Pineda, José S Delgado, Irmgard Montfort, Ruy Pérez-Tamayo
New insights into amoeba-bacteria
relationship
Felipe Padilla-Vaca*, Claudia Mendoza-Macías, Minerva
Barrios-Ceballos, Ángeles Rangel-Serrano, Fernando Anaya-Velázquez, Rivka
Bracha, David Mirelman
Expression and localization of gal/galnac in the virulent and non virulent variants from E. histolytica HM1:IMSS
Rosario López
Vancell*, Roberto Arreguín Espinosa, Mario Néquiz Avendaño, Augusto González Canto,
Dora López Vancell, Ruy Pérez Tamayo
Study of the encystment process in Entamoeba invadens
Norma Cristina Segovia-Gamboa*, Yolanda
Medina-Flores, Verónica Ivonne Hernández-Ramírez, Bibiana Chávez-Munguía,
Patricia Talamás-Rohana
The lysine and glutamic acid-rich protein 1 (KERP1) is involved in the early steps of amoebic liver abscess development
Julien
Santi-Rocca*, Nancy Guillén
Discussion
(19:00 - 19:30)
Scientific Program
Session III A : Posters 1
to 7 (17:30 - 19:30)
Immunology:
Vaccines, Cytokine Production
Chairpersons: Adolfo
Martínez-Palomo, Kris Chadee, Blanca Sánchez, Jonathan Ravdin
Evaluation of immunogenicity and protective
efficacy OFA pcDNA-EhCPADH vaccine in hamster
Máximo B. Martínez*, Mario A. Rodríguez, Guillermina García-Rivera, Tomás Sánchez, Rogelio Hernández-Pando, Diana Aguilar, Esther Orozco
Construction
and evaluation of two T polyepitopes from the EhCPADH complex of Entamoeba histolytica in hamsters
Jonnatan
Pais*, Máximo B. Martínez, Esther Orozco
Protein tyrosine
phosphatase from Entamoeba histolytica modulates
the secretion of cytokines and translocation of nuclear factor NFkB from macrophages
Juan Espejel-Zaragoza,
Alma Escalona-Montaño, Mario Nequiz, Amelia Angel, Laila Gutiérrez-Kobeh, Ruy
Pérez-Tamayo, Patricia Talamás-Rohana, María Magdalena Aguirre-García*
Cytokine production in leukocytes of patients
from ALA exposed to the monocyte locomotion inhibitory factor produced by Entamoeba histolytica
Sara
Rojas-Dotor*, Patricia Morán Silva, Cecilia Ximénez García, Guadalupe
Rico Rosillo
Expression of immune modulator cytokines in human fulminant amebic colitis
Javier
Ventura-Juárez*, RE. Sierra-Puente, R. Campos-Rodríguez,
RA. Jarillo-Luna, L. Muñoz-Fernández
Expression
of prostaglandin EP2 and EP4 receptors during cyclooxygenase-2 induction in
macrophages stimulated with Entamoeba histolytica antigens
Hiriz
Chaparro-Reyes*, Gabriela Sosa-Robledo, Carmen González Horta, Gilberto Erosa
De La Vega, Patricia Talamás Rohana, Blanca Sánchez Ramírez
Expression of prostaglandin EP2 and EP4 receptors during amoebic liver abscess formation in hamsters
Celia Quiñones
Flores*, Ana Rosa Hernández Garibay, Ernesto Ramos Martínez, Carmen González
Horta, Gilberto Erosa De La Vega, Porfirio Gutiérrez Martínez, Patricia Talamás
Rohana, Blanca Sánchez Ramírez
Discussion
(19:00 - 19:30)
Session
Experimental Amebiasis
Chairpersons: Sarah A Evans, Mineko Shibayama, Easwaran Variyam
Kinetics of cytokines production
in the amoebic liver abscess of hamster
José Manuel Galván Moroyoqui*, Judith Pacheco-Yépez,
Víctor Tsutsumi, Isaura Meza, Mineko Shibayama
Entamoeba histolytica induces apoptosis in both inflammatory cells and hepatocytes using
hamster model
José D¥Artagnan Villalba-Magdaleno*, Silvia
Galindo Gómez, Guillermo Pérez Ishiwara, Mineko Shibayama
A Brazilian species of Entamoeba dispar produces amoebic liver abscess with an important protease activity
Silvio Dolabella*,
Edward
Silva, Jesús Serrano-Luna, Víctor Tsutsumi, Mineko Shibayama
Morphometric changes in
Entamoeba histolytica trophozoites
present in liver abscess in misoprostol- treated BALB/c mice
Blanca
Sánchez-Ramírez*, Andrés Aceves-Cano, Carmen González-Horta, Margarita
Levario-Carrillo, Blanca Rivera-Chavira, Rocío Infante-Ramírez, Patricia
Talamás-Rohana
Prolonged noninvasive intestinal amebiasis in gnotobiotic rat
Easwaran P. Variyam*, Gordon Brackee
Involvement of IL-4, IFN1g, TNF-a and cyclophilin in the development of the amoebic liver abscess
María del Pilar Crisóstomo-Vázquez *, Leticia Eligio García., Victor A. Maravélez Acosta, Jorge Hernández-García, Andrés Flores-Luna, Enedina Jiménez-Cardoso.
Discussion
(19:00 - 19:30)
Session
Therapeutics
Chairpersons: Michael
Duchêne, Maria Porfiria Barrón, Iris Bruchhaus
In
vitro antiamoebic activities of fractions from a
medical plant from
Flore
Melanie *, Hubert Donfack, Emmanuel Njoya, Moundipa Paul
Bilong-Bilong, Egbert
Tannich, Iris Bruchhaus
In vitro and toxicity studies of Codiaeum variegatum
on Entamoeba histolytica.
Gondame Flore Mélanie *, Paul Moundipa Fewou, Bilong-Bilong, CF, I.
Bruchhaus
Extracts from marine actinomycetes as novel anti amebic agents
Erica Ryke, Aaron Socha, David Rowley, Avelina Espinosa*
Antiamoebic effect of
methanolic extracts from four species of family cactacea
Eufemia Morales
R.*, Jaime Treviño N., Ruth Garza P., Mario Morales V., Azucena Oranday C.,
María Porfiria Barrón G.
Determination
of the inhibitory concentration 50% (IC50) of methanolic extracts of
Ariocarpus kotschoubeyanus, Ariocarpus retusus and Fouquieria splendens on axenic growth of Entamoeba histolytica
Ramón
Gerardo Rodríguez Garza*, María Eufemia Morales Rubio, María Julia Verde Star,
Ruth Amelia Garza Padrón, Mario R. Morales Vallarta, María Porfiria Barrón
González
Amoebicid activity
evaluation of extract astrophytum
myriostigma stem and in vitro callus
Ruth Amelia Garza
Padrón*, Ma. Porfiria Barrón González, Ma. Julia Verde Star, Ma. Eufemia
Morales Rubio, Ramón Gerardo Rodríguez Garza, Mario Morales Vallarta
Phagocytosis assays with Entamoeba
histolytica as a model system to define biocompatibility of multiwalled
carbon nanotubes
Julio C. Carrero*, Ana L. Elías,
Humberto Terrones, Morinobu Endo, Mauricio Terrones, Juan P. Laclette
Discussion
(19:00 - 19:30)
Session III D: Posters 21
to 27Ý (17:30 - 19:30)
Epidemiology and
Diagnostic Tools
Chairpersons: Ji Dar-Der,
William Petri Jr.,Christen Stensvold, Cecilia Ximénez
Infection frequency of Entamoeba species in a turtle collection
in
Rodrigo Gutiérrez*, Gabriela García, Alicia Valadéz, Fernando Ramos,
Monica Salmerón, Patricia Morán, Enrique González, Jorge Yañez, Paul Gaytán,
Cecilia Ximénez
Differentiation
between Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using
pyrosequencing and prevalence analysis of Entamoeba
moshkovskii in patients positive for the Entamoeba complex diagnosed in Northwestern Europe
Christen R.
Stensvold*, Marianne Lebbad, Jaco J. Verweij, Venkata R. S. Geedi, J¯rgen S.
Jensen, Cathrine Jespersgaard, Georg von Samson-Himmelstjerna, Henrik V.
Nielsen
Isolation and identification of Acanthamoeba species related to amebic
encephalitis and non-pathogenic Vannella species
in
Liang Shih-Yu *, Ji Dar-Ren, Hsia Kan-Tai, Hsu Bing-Mu, Chen Jung-Sheng, Wu Ming-Huei, Lai Cheng-Hao, Ji Dar-Der
The heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity
Kyoung-Ju Song*
Immunodominant low molecular weight
recombinant protein of Entamoeba
histolytica expressed by Escherichia.
coli
Dinora Pérez*, R.
Rangel, A. Rojo, G. Mendoza, B. González, D. Barrientos J. Viader, E. Tamez, R.
Tijerina, F. Bosques, A. Isibasi, L. Galán, K. Arévalo, MS. Flores
PCR amplification of different DNA regions
of Entamoeba histolytica species
performed in amebic or pyogenic liver abscesess in
Liliana Rojas*, Linda García, Olivia Valenzuela, Fernando Ramos, Patricia Morán, Gabriela García, Alicia Valadez, Enrique González, Angélica Limón, Erick Hernandez, Cecilia Ximénez
Development of loop-mediated isothermal
amplification assay for diagnosis of Entamoeba
histolytica
Ji Dar-Der *,
Liang Shih-Yu, Chan Yun-Hsien, Hsia Kan-Tai, Lee Jing-Lun, Kuo Ming-Chu, Hwa
Kuo-Yuan, Chan Chi-Wen, Chiang Ting-Yi, Chen Jung-Sheng, Wu Fang-Tzy
Discussion
(19:00 - 19:30)