The fragment comigration data derived from riboprint patterns were converted into estimated genetic distances by the method of Nei and Li and the tree constructed by the Fitch-Margoliash method (panel A). The total tree standard deviation for the 4-nucleated cyst producing species is 10.9%, one third of which is due to E. moshkovskii Ribodeme 4. Panel B shows a tree derived from the fragment comigration data using parsimony (MacClade software).