Louis S. Diamond

Full Name:  Louis Stanley Diamond
Place of Birth:   Philadelphia, Pennsylvania
Date of Birth:   February 6th, 1920
Education:   A.B., University of Pennsylvania, 1940
M.S., University of Michigan, 1941
Ph.D., University of Minnesota, 1958
Employment: U.S. Department of the Interior,
Fish and WildlifeService, 1951-1953
U.S. Department of Agriculture,
Animal Disease and Parasite Research Division, 1953-1959
U.S. National Institutes of Health,
Laboratory of Parasitic Diseases, 1959-1994
Research Landmarks: 1957 - TYM
The most widely used medium for the axenic cultivation of trichomonads
1961 - First axenic cultivation of Entamoeba histolytica
1964 - Cryopreservation and recovery of protozoa from liquid nitrogen
1968 - TPS-1
First monophasic medium for the axenic cultivation of Entamoeba histolytica
1972 - Viruses of Entamoeba histolytica
1978 - TYI-S-33
The most widely used medium for the axenic cultivation of Entamoeba histolytica
1989 - Description of ribosomal DNA plasmids in Entamoeba histolytica
1993 - Redescription of Entamoeba histolytica to distinguish it from E. dispar

Selected Publications in Amoebiasis

Diamond, L.S. (1961) Axenic cultivation of Entamoeba histolytica. Science 134: 336-337.

Diamond, L.S. (1964) Freeze-preservation of protozoa. Cryobiology 1: 95-102.

Diamond, L.S. (1968) Improved method for the monoxenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae with trypanosomatids. J. Parasitol. 54: 715-719.

Diamond, L.S. (1968) Techniques of axenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae. J. Parasitol. 54: 1047-1056.

Lunde, M.N. & Diamond, L.S. (1969) Studies on antigens from axenically cultivated Entamoeba histolytica and Entamoeba histolytica-like amebae. Am. J. Trop. Med. Hyg. 18: 1-6.

Gelderman, A.H., Bartgis, I.L., Keister, D.B. & Diamond, L.S. (1971) A comparison of genome sizes and thermal denaturation-derived base composition of DNAs from several members of Entamoeba (histolytica group). J. Parasitol. 57: 912-916.

Diamond, L.S., Mattern, C.F.T. & Bartgis, I.L. (1972) Viruses of Entamoeba histolytica I. Identification of transmissible virus-like agents. J. Virol. 9: 326-341.

Weinbach, E.C. & Diamond, L.S. (1974) Entamoeba histolytica: I. Aerobic metabolism. Exp. Parasitol. 35: 232-243.

Diamond, L.S., Harlow, D.R. & Cunnick, C.C. (1978) A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans. R. Soc. Trop. Med. Hyg. 72: 431-432.

Gillin, F.D. & Diamond, L.S. (1978) Clonal growth of Entamoeba histolytica and other species of Entamoeba in agar. J. Protozool. 25: 539-543.

Diamond, L.S. (1982) A new liquid medium for xenic cultivation of Entamoeba histolytica and other lumen dwelling protozoa. J. Parasitol. 68: 958-959.

Diamond, L.S. (1983) Lumen dwelling protozoa: Entamoeba, trichomonads, and Giardia. In J. B. Jensen (Eds.), In vitro cultivation of protozoan parasites. Boca Raton, FL: CRC Press, pp. 67-109.

Diamond, L.S. (1986) Entamoeba histolytica Schaudinn, 1903: from xenic to axenic cultivation. J. Protozool. 33: 1-5.

Bhattacharya, S., Bhattacharya, A. & Diamond, L.S. (1987) Comparison of repeated DNA from strains of Entamoeba histolytica and other Entamoeba. Mol. Biochem. Parasitol. 27: 257-262.

Bhattacharya, S., Bhattacharya, A., Diamond, L.S. & Soldo, A.T. (1989) Circular DNA of Entamoeba histolytica encodes ribosomal RNA. J. Protozool. 36: 455-458.

Clark, C.G. & Diamond, L.S. (1991) Ribosomal RNA genes of 'pathogenic' and 'nonpathogenic' Entamoeba histolytica are distinct. Mol. Biochem. Parasitol. 47: 297-302.

Diamond, L.S. & Cunnick, C.C. (1991) A serum-free, partly defined medium, PDM-805, for the axenic cultivation of Entamoeba histolytica Schaudinn, 1903 and other Entamoeba. J. Protozool. 38: 211-216.

Diamond, L.S. & Clark, C.G. (1993) A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. J. Euk. Microbiol. 40: 340-344.

Diamond, L.S. (1995) Cryopreservation and storage of parasitic protozoa in liquid nitrogen. J. Euk. Microbiol. 42: 585-590.

Diamond, L.S., Clark, C.G. & Cunnick, C.C. (1995) YI-S, a casein-free medium for axenic cultivation of Entamoeba histolytica, related Entamoeba, Giardia intestinalis and Trichomonas vaginalis. J. Euk. Microbiol. 42: 277-278.

Clark, C.G. & Diamond, L.S. (1997) Intraspecific variation and phylogenetic relationships in the genus Entamoeba as revealed by riboprinting. J. Euk. Microbiol. 44: 142-154.

Clark, C.G. & Diamond, L.S. (2002) Methods for cultivation of luminal parasitic protists of clinical importance. Clin. Microbiol. Rev. 15: 329-341.


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