Amebiasis Newsletter

Editors: C. G. Clark, LSHTM, London, England, L. S. Diamond, NIH, Bethesda MD, USA,

M. Espinosa Cantellano, A. Martínez-Palomo, CINVESTAV-IPN, México DF

Nº 15, Autumn 1997

Correspondence should be sent to:

Dr. C. Graham Clark, Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, England, G.B.

OR: Dra. Martha Espinosa Cantellano, Departamento de Patología Experimental, CINVESTAV- IPN, Aptdo. Postal 14-740, CP 07000 México D.F., México

Entamoeba on the World Wide Web

As mentioned in the last Newsletter, a website for Entamoeba researchers has been developed. The content of the site is far from completed but the information is now accessible at the following location:

The site contains basic information about Entamoeba histolytica, information on its cultivation and other methods, addresses and contact information for amebiasis researchers, links to other sites with amebiasis information and graphics, links to relevant journals, reference lists, and recent issues of this Newsletter, among other files.

All comments will be gratefully received as would additional links to appropriate web pages, graphics files, and any other useful material.

Recent Publications in Amebiasis

Adou-Bryn, K.D., Enoh, J.E.S., Ouhon, J., Kassi, E.A., Assomou, A. & Kone, M. (1997) [Findings of parasitologic examinations of stools over a five year period in Abidjan, Côte d'Ivoire (in French)]. Méd. Trop. 57: 206-207.

Albonico, M., Shamlaye, N., Shamlaye, C. & Savioli, L. (1996) Control of intestinal parasitic infections in Seychelles: a comprehensive and sustainable approach. Bull. Wld. Hlth. Org 74: 577-586.

Anand, A.C. (1997) Is it curtains for chronic amebiasis? Indian J. Gastroenterol . 16: 127-129.

Ankri, S., Miron, T., Rabinkov, A., Wilchek, M. & Mirelman, D. (1997) Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica. Antimicrob. Agents Chemother. 41: 2286-2288.

Anonymous (1997) Entamoeba taxonomy. Bull. Wld. Hlth. Org. 75: 291-292.

Anonymous (1997) WHO/PAHO/UNESCO report. A consultation with experts on amoebiasis. Epidemiol. Bull. PAHO 18: 13-14.

Bailen, L.S. & Peppercorn, M.A. (1997) Sexually transmitted anorectal infections. In J. T. LaMont (Eds.), Gastrointestinal infections: diagnosis and management. New York: Marcel Dekker, Inc., pp. 411-451.

Berninghausen, O. & Leippe, M. (1997) Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621.

Bruchhaus, I., Richter, S. & Tannich, E. (1997) Removal of hydrogen peroxide by the 29kDa protein of Entamoeba histolytica. Biochem. J. 326: 785-789.

Cabrera, N., Rangel, P., Hernandez Munoz, R. & Perez Montfort, R. (1997) Purification of alcohol dehydrogenase from Entamoeba histolytica and Saccharomyces cerevisiae using zinc-affinity chromatography. Prot. Expr. Purif. 10: 340-344.

Cafasso, O.T., Garcia, S.H., Prat, M.I. & Santamaria, B. (1996) [Canine enteroparasites in a sector of Bahia Blanca, Argentina (in Spanish)]. Parasitol. al Dia 20: 144-146.

Clark, C.G. (1997) Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Euk. Microbiol. 44: 277-283.

Devani, S. & Travis, S.P.L. (1997) Intestinal and hepatic amoebiasis in a sailor. Illustr. Case Reports Gastroenterol. 4: 53-59.

Doganci, L., Tanyuksel, M. & Gun, H. (1997) Overdiagnosis of intestinal amoebosis in Turkey (letter). Lancet 350: 670.

Drabick, J.J., Gambel, J.M., Huck, E., De Young, S. & Hardeman, L. (1997) Microbiological laboratory results from Haiti: June-October 1995. Bull. Wld. Hlth. Org 75: 109-115.

Eichinger, D. (1997) Encystation of Entamoeba parasites. Bioessays 19: 633-639.

Fätkenheuer, G., Arnold, G., Steffen, H.-M., Franzen, C., Schrappe, M., Diehl, V. & Salzberger, B. (1997) Invasive amoebiasis in two patients with AIDS and cytomegalovirus colitis. J. Clin. Microbiol. 35: 2168-2169.

Freeman, C.D., Klutman, N.E. & Lamp, K.C. (1997) Metronidazole. A therapeutic review and update. Drugs 54: 679-708.

Gakiya, I., Kugai, Y., Hayashi, S., Nimura, S., Zaha, O., Kouchi, A., Oshiro, J., Sakugawa, H., Kitukawa, K., Kinjou, F., Saitou, A. & Araki, K. (1997) Varioliform mucosal polypoid lesions in intestinal tract in a patient with adult T-cell leukemia. J. Gastroenterol. 32: 553-557.

Gangopadhyay, S.S., Sankar Ray, S., Sinha, P. & Lohia, A. (1997) Unusual genome organisation in Entamoeba histolytica leads to two overlapping transcripts. Mol. Biochem. Parasitol. 89: 73-83.

Gatti, S., Cevini, C., Bruno, A., Ramsan, M., Marchi, L. & Scaglia, M. (1997) First isolation and characterization in humans of Entamoeba histolytica (laboratory-made) zymodeme XX. Parasitol. Res. 83: 716-718.

Ghosh, S.K. & Samuelson, J. (1997) Involvement of p21racA, phosphoinositide 3-kinase, and vacuolar ATPase in phagocytosis of bacteria and erythrocytes by Entamoeba histolytica: suggestive evidence for coincidental evolution of amebic invasiveness. Infect. Immun. 65: 4243-4249.

Gomes, M.A., Melo, M.N., Pena, G.P.M. & Silva, E.F. (1997) Virulence parameters in the characterization of strains of Entamoeba histolytica. Rev. Inst. Med. Trop. São Paulo 39: 65-69.

Gomez, M.S., Gracenea, M., Montoliu, I., Feliu, C., Monleon, A., Fernandez, J. & Ensenat, C. (1996) Intestinal parasitism - protozoa and helminths - in primates at the Barcelona Zoo. J. Med. Primatol. 25: 419-423.

Gopal, B., Swaminathan, C.P., Bhattacharya, S., Bhattacharya, A., Murthy, M.R.N. & Surolia, A. (1997) Thermodynamics of metal ion binding and denaturation of a calcium binding protein from Entamoeba histolytica. Biochemistry 36: 10910-10916.

Gopanpallikar, A., Rathi, P., Sawant, P., Gupta, R., Dhadphale, S. & Deshmukh, H.L. (1997) Hepatic artery pseudoaneurysm associated with amebic liver abscess presenting as upper GI hemorrhage. Am. J. Gastroenterol. 92: 1391-1393.

Guarner, J., Matilde Nava, T., Villasenor Flores, R. & Sánchez Mejorada, G. (1997) Frequency of intestinal parasites in adult cancer patients in Mexico. Arch. Med. Res. 28: 219-222.

Gupta, R.K. (1997) Generic prescription and anti-amoebic drugs (letter). Natl. Med. J. India 10: 152.

Hohenschild, S. (1997) Diagnosing amoebiasis (Letter). Lancet 350: 1034.

Horta, M.F. (1997) Pore-forming proteins in pathogenic protozoan parasites. Trends Microbiol. 5: 363-366.

Im, K.I., Choi, J.T., Hong, Y.P., Kim, T.E. & La, M.S. (1997) [cDNAs encoding the antigenic proteins in pathogenic strain of Entamoeba histolytica (in Korean)]. Korean J. Parasitol. 35: 203-210.

Jiva, T.M. (1997) Systemic manifestations are common in long-term infection: Is a parasite causing your patient's chronic lung disease? J. Respir. Dis. 18: 603-617.

Kelly, J.M. (1997) Genetic transformation of parasitic protozoa. Adv. Parasitol. 39: 227-270.

Kim, J.M., Jung, H.C., Jin, D.Z., Im, K.I., Song, I.S. & Kim, C.Y. (1997) Cytokine genes are down-regulated when attachment of Entamoeba histolytica to HT-29 colon epithelial cells is prevented. Scand. J. Immunol. 45: 613-617.

Kimura, K., Stoopen, M., Reeder, M.M. & Moncada, R. (1997) Amebiasis: modern diagnostic imaging with pathological and clinical correlation. Semin. Roentgenol. 32: 250-275.

Landa, A., Rojo Dominguez, A., Jimenez, L. & Fernandez Velasco, D.A. (1997) Sequencing, expression and properties of triosephosphate isomerase from Entamoeba histolytica. Eur. J. Biochem. 247: 348-355.

Laraqui, C., Belamallem, I., Harourate, K., Joutey, N.A., Ben Haymoud, N., Verger, C. & Curtes, J.P. (1996) [Study of occupational hazards in the Rabat tannery (in French)]. Arch. Mal. Profess. Med. Travail. 57: 200-204.

Lecuit, M., Martinez, F., Deray, G., Beaufils, H., Gubler, M.C., Nozais, J.P., Bricaire, F. & Jacobs, C. (1997) Clinical and pathophysiological aspects of immune complex glomerulonephritis associated with Entamoeba histolytica abscess of the liver. Clin. Infect. Dis. 25: 335-336.

Loschiavo, F., Guarneri, B., Ventura Spagnolo, T., D'Ambrosio, F., P., Ricciardi, L. & Cannavo, S.P. (1997) Cutaneous amebiasis in an Iranian immunodeficient alcoholic: Immunochemical and histological study (Letter). Dermatology 194: 370-371.

Lotter, H. & Tannich, E. (1997) The galactose-inhibitable surface lectin of Entamoeba histolytica, a possible candidate for a subunit vaccine to prevent amoebiasis. Behring Inst. Mitt. 99: 112-116.

Lyche, K.D. & Jensen, W.A. (1997) Pleuropulmonary amebiasis. Semin. Respir. Infect. 12: 106-112.

Mahanta, J., Narain, K. & Srivastava, V.K. (1996) Intestinal parasitic infestation in a rural population of Upper Assam. J. Parasit. Dis. 20: 57-58.

Marecki, M., Heeb, K. & Zitka, W. (1997) Protozoan infection in the perinatal period. J. Perinat. Neonatal. Nurs. 11: 21-33.

Marinets, A., Zhang, T., Guillén, N., Gounon, P., Bohle, B., Vollmann, U., Scheiner, O., Wiedermann, G., Stanley, S.L. & Duchêne, M. (1997) Protection against invasive amebiasis by a single monoclonal antibody directed against a lipophosphoglycan antigen localized on the surface of Entamoeba histolytica. J. Exp. Med. 186: 1557-1565.

Mazzuco, A., Benchimol, M. & De Souza, W. (1997) Endoplasmic reticulum and Golgi-like elements in Entamoeba. Micron 28: 241-247.

Mendoza, M.T., Liquete, R.R., Ona, E.T. & Alano, F.A. (1997) Infections in renal allograft recipients: A review of the Philippine experience. Int. J. Infect. Dis. 1: 222-225.

Mirelman, D., Nuchamowitz, Y. & Stolarsky, T. (1997) Comparison of use of enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes for simultaneous detection of Entamoeba histolytica and E. dispar. J. Clin. Microbiol. 35: 2405-2407.

Monge, R. & Arias, M.L. (1996) [Occurrence of some pathogenic microorganisms in fresh vegetables in Costa Rica (in Spanish)]. Arch. Latinoamer. Nutr. 46: 292-294.

Monge, R., Chinchilla, M. & Reyes, L. (1996) [Occurrence of parasites and intestinal bacteria in vegetables that are consumed raw in Costa Rica (in Spanish)]. Rev. Biol. Trop. 44: 369-375.

N'Gbesso, R.D. & Keita, A.K. (1997) [Ultrasound study of amebic liver abscesses: a new classification (in French)]. J. Radiol. 78: 569-576.

Nawaz, Y. & Nawaz, M. (1996) Gastrointestinal parasitic infections in Uzbek tribe, a Central Asian race settled in Quetta, Pakistan. Hamdard Medicus 39: 105-109.

Nayak, A.K., Karnad, D.R., Abraham, P. & Mistry, F.P. (1997) Metronidazole relieves symptoms in irritable bowel syndrome: the confusion with so-called 'chronic amebiasis'. Indian J. Gastroenterol. 16: 137-139.

Nierhaus, A. (1997) [Complicated amoebic liver abscess - Case report (in German)]. Anästhesiologie Intensivmedizin Notfallmedizin Schmerztherapie 32: 518-521.

Oyofo, B.A., Peruski, L.F., Ismail, T.F., El Etr, S.H., Churilla, A.M., Wasfy, M.O., Petruccelli, B.P. & Gabriel, M.E. (1997) Enteropathogens associated with diarrhea among military personnel during Operation Bright Star 96, in Alexandria. Egypt. Military Med. 162: 396-400.

Palau, L.A. & Kemmerly, S.A. (1997) First report of invasive amebiasis in an organ transplant recipient. Transplantation 64: 936-937.

Poole, L.B., Chae, H.Z., Flores, B.M., Reed, S.L., Rhee, S.G. & Torian, B.E. (1997) Peroxidase activity of a TSA-like antioxidant protein from a pathogenic amoeba. Free Radical Biol. Med. 23: 955-959.

Ramos, M.A., Mercado, G., C,, Salgado, L.M., Sanchez-Lopez, R., Stock, R.P., Lizardi, P.M. & Alagón, A. (1997) Entamoeba histolytica contains a gene encoding a homologue to the 54kDa subunit of the signal recognition particle. Mol. Biochem. Parasitol. 88: 225-235.

Renesto, P., Sansonetti, P.J. & Guillén, N. (1997) Interaction between Entamoeba histolytica and intestinal epithelial cells involves a CD44 cross-reactive protein expressed on the parasite surface. Infect. Immun. 65: 4330-4333.

Ribeiro, S., de Araujo Soares, R.M., Sales Alviano, C., Da Silva, E.F., De Souza, W. & Angluster, J. (1997) Cell-surface carbohydrates of Entamoeba invadens. Parasitol. Res. 83: 801-805.

Rockey, D.C. (1997) Hepatobiliary infections. Curr. Opin. Gastroenterol. 13: 216-220.

Ryan, E.T., Butterton, J.R., Zhang, T., Baker, M.A., Stanley, S.L., Jr. & Calderwood, S.B. (1997) Oral immunization with attenuated vaccine strains of Vibrio cholerae expressing a dodecapeptide repeat of the serine-rich Entamoeba histolytica protein fused to the cholera toxin B subunit induces systemic and mucosal antiamebic and anti-V. cholerae antibody responses. Infect. Immun. 65: 3118-3125.

Sabanero, G.B., Duran, G.C., Hernandez, M., Torres, H. & Sabanero, M. (1996) Analysis of detergent-resistant cytoskeleton from Entamoeba histolytica. Biomed. Letts. 54: 183-191.

Sachdev, G.K. & Dhol, P. (1997) Colonic involvement in patients with amebic liver abscess: Endoscopic findings. Gastrointestinal Endoscopy 46: 37-39.

Safar, E.H., Shady, O.M.A., El Adawi, A.J., Khattab, H.M. & Gawdat, S.S. (1996) Intestinal parasitic infections in immunosuppressed patients. J. Egypt. Soc. Parasitol. 26: 639-643.

Saidi, S.M., Iijima, Y., Sang, W.K., Mwangudza, A.K., Oundo, J.O., Taga, K., Aihara, M., Nagayama, K., Yamamoto, H., Waiyaki, P.G. & Honda, T. (1997) Epidemiological study on infectious diarrheal diseases in children in a coastal rural area of Kenya. Microbiol. Immunol. 41: 773-778.

Sanchez Ramirez, B., Escalante, B., Rosales Encina, J.L. & Talamas Rohana, P. (1997) Role of prostaglandin E-2 on amoebic liver abscess formation in hamsters. Prostaglandins 53: 411-421.

Schlosser, O. (1997) [Intestinal parasitoses in staff exposed to waste water (in French)]. Arch. Maladies Prof. Méd. Travail 58: 377-379.

Seydel, K.B., Zhang, T. & Stanley, S.L., Jr. (1997) Neutrophils play a critical role in early resistance to amebic liver abscesses in severe combined immunodeficient mice. Infect. Immun. 65: 3951-3953.

Singh, B., Moodley, J., Ramdial, P. & Haffejee, A.A. (1997) Ileal amoebiasis. J. Royal Coll. Surg. Edinburgh 42: 200-202.

Singh, U. & Petri, W.A., Jr. (1997) Molecular medicine's applications to the developing world: enlightened self interest. Mol. Med. Today 3: 234-235.

Singh, U., Rogers, J.B., Mann, B.J. & Petri, W.A., Jr. (1997) Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica. Proc. Natl. Acad. Sci. USA 94: 8812-8817.

Sinha, P., Ghoshal, U.C., Choudhuri, G., Naik, S., Ayyagari, A. & Naik, S.R. (1997) Does Entamoeba histolytica cause irritable bowel syndrome? Indian J. Gastroenterol. 16: 130-133.

Sinha, P., Naik, S., Ayyagari, A. & Naik, S.R. (1997) The effect of in vitro bacterial association on virulence of Entamoeba histolytica. Indian J. Med. Res. 105: 266-270.

Stanley, S.L., Jr. (1997) Progress towards development of a vaccine for amebiasis. Clin. Microbiol. Rev. 10: 637-649.

Stott, R., Jenkins, T., Shabana, M. & May, E. (1997) A survey of the microbial quality of waste waters in Ismailia, Egypt and the implications for wastewater reuse. Water Sci. Technol. 35: 211-217.

Tachibana, H., Kobayashi, S., Kaneda, Y., Takeuchi, T. & Fujiwara, T. (1997) Preparation of a monoclonal antibody specific for Entamoeba dispar and its ability to distinguish E. dispar from E. histolytica. Clin. Diagn. Lab. Immunol. 4: 409-414.

Tai, E.S. & Fong, K.Y. (1997) Fatal amoebic colitis in a patient with SLE: a case report and review of the literature. Lupus 6: 610-612.

Tanaka, T., Tanaka, M. & Mitsui, Y. (1997) Analysis of expressed sequence tags (ESTs) of the parasitic protozoa Entamoeba histolytica. Biochem. Biophys. Res. Commun. 236: 611-615.

Tarimo, D.S., Killewo, J.Z.J., Minjas, J.N. & Msamanga, G.I. (1996) Prevalence of intestinal parasites in adult patients with enteropathic AIDS in north-eastern Tanzania. East Afr. Med. J. 3: 397-399.

Te Velde, L.F., Blaauwiekel, E.E., Sprenger, H.G. & Weits, J. (1997) [An amoebic abscess in the liver: early suspicion, late detection (in Dutch)]. Ned. Tijdschr. Geneeskd. 141: 1436-1439.

Thammapalerd, N., Sherchand, J.B., Kotimanusvanij, D., Chintana, T. & Tharavanij, S. (1996) Monoclonal antibody-based ELISA for the detection of circulating Entamoeba histolytica antigen in hepatic amebiasis in hamsters (Mesocricetus auratus). S. E. Asian J. Trop. Med. Publ. Hlth. 27: 760-764.

Triadafilopoulos, G. (1997) Parasitic infections of the intestine. In J. T. LaMont (Eds.), Gastrointestinal infections: diagnosis and management. New York: Marcel Dekker, Inc., pp. 327-360.

Van Ha, M. (1997) Some peculiarities of hepatobiliary diseases in Vietnam. J. Gastroenterol. Hepatol. 12: S15-S18.

Vargas, M., Voigt, H., Sansonetti, P. & Guillen, N. (1997) Molecular characterization of myosin IB from the lower eukaryote Entamoeba histolytica, a human parasite. Mol. Biochem. Parasitol. 86: 61-73.

Walderich, B., Mueller, L., Bracha, R., Knobloch, J. & Burchard, G.D. (1997) A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples. Parasitol. Res. 83: 719-721.

Wasilewska, E., Propokowicz, D., Kondej Muszynska, K. & Trippner, M. (1996) [Liver and bile duct pathology in the course of parasitoses in man and animals (in Polish)]. Zycie Weterynaryjne 71: 175-178.

Younes, T.A., Hussein, M.M., Kamal, S.M., Mahmoud, D.M. & Metwaly, S.F. (1996) Parasitological and bacteriological studies in recurrent diarrhoea in patients with chronic liver diseases. J. Egypt. Soc. Parasitol. 26: 697-708.

Zhang, T.H. & Stanley, S.L. (1997) Expression of the serine rich Entamoeba histolytica protein (SREHP) in the avirulent vaccine strain Salmonella typhi TY2 [chi] 4297 ([Delta] cya [Delta] crp [Delta] asd): safety and immunogenicity in mice. Vaccine 15: 1319-1322.

Return to Amebiasis Newsletters