Scientific Program
Session I (9:00 - 11:00) Room Camino Real
Genome
Organization and Gene expression
Chairpersons: Upinder
Singh and Mario A. Rodríguez
9:00 - 9:15 From
Ayala Tovy, Tal Lavi, Sophie Katz, Rama Siman Tov, Serge Ankri*
9:15 - 9:30 Entamoeba
histolytica high-mobility-group box protein which is induced during
intestinal infection is an architectural Mayuresh M. Abhyankar*, Amelia E.
Hochreiter, Jessica Hershey, Clive Evans, Yan Zhang, Oswald Crasta, Bruno W. S.
Sobral, Barbara J. Mann, William A. Petri Jr., Carol A. Gilchrist 9:30 - 9:45 Expression and
cellular localization of EhRAD54
recombinase in response to Lorena García-Morales*, Cesar López-Camarillo, Mavil
López-Casamichana, Esther Orozco, Laurence A. Marchat 9:45 - 10:00 Single
nucleotide polymorphism analysis of population structure and virulence in Entamoeba
histolytica Gareth D. Weedall*, C. Graham Clark, Steve Paterson, Neil
Hall 10:00-10:15 Delimitation
of the cis-element that negatively
regulates the transcription of the EhRABB gene of Entamoeba histolytica Jenny Gómez-Sandoval*, Mario Hernández-Alejandro,
Mercedes Calixto-Gálvez, Consuelo Gómez, Mario A. Rodríguez 10:15-10:30 EuPathDB
and the amebiasis research community. David S. Roos* 10:30 - 11:00 Discussion 11:00-11:30 COFFEE BREAK Session II (11:30 - 13:30) Room Camino Real Gene Transcription Chairpersons: David
Mirelman and Guillermo Pérez-Ishiwara 11:30 - 11:45 Targets of the Entamoeba
histolytica transcription factor URE3‚BP
Carol A.
Gilchrist*, Yan Zhang, Oswald Crasta, Clive Evans, Elisabet Caler, Bruno W.S.
Sobral, Barbara J. Mann, William A. Petri Jr. 11:45 - 12:00 Small RNAs with 5¢-polyphosphate
termini associate with a piwi-related protein and regulate gene expression in
the single celled eukaryote Entamoeba
histolytica Hanbang Zhang, Gretchen
M. Ehrenkaufer, Justine M. Pompey, Jason A. Hackney, Upinder Singh * 12:00 - 12:15 Transcriptional silencing of the
rhomboid (EhROM1) protease in the amoebapore-silenced Entamoeba histolytica G3 David Mirelman*, Rivka Bracha, Leigh A.
Baxt, Upinder Singh 12:15 - 12:30 Pre-ribosomal
RNA accumulation in growth-stressed Entamoeba
histolytica cells: a novel regulatory mechanism Sunil K. Panigrahi, Gagan Deep Jhingan, Abhishek Gupta, Sudha
Bhattacharya* 12:30 - 12:45 Structural
characterization and expression analysis of the ying yang 1-like transcription
factor Lilia Y. Tapia-Chávez, César,
S. López, Olivia Medel-Flores, Elvira Náder, D. Guillermo Pérez, Consuelo Gómez* 12:45 - 13:00 Short
hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica Alicia
S. Linford*, Heriberto Moreno, Katelyn R. Good, Hanbang Zhang, Upinder Singh,
William A. Petri, Jr 13:00 - 13:30 Discussion 13:30 - 15:00 LUNCH
Session III
(15.00 - 16:00) Room Camino Real Protein Structure A Chairpersons: Tomoyoshi
Nozaki and Iris Bruchhaus 15:00 - 15:15 Structure
and mutational analysis from Entamoeba
histolytica EhGEF1
protein Nora Adriana Hernández-Cuevas*, Arturo
Rojo-Domínguez, M.de Jesús Almaraz- Barrera, Miguel Vargas 15:15 - 15:30 Role
of sulfur-containing amino acid metabolism: characterization of two isotypes of
methionine g-lyase in Entamoeba histolytica Tomoyoshi Nozaki*, Atsushi Furukawa, Ghulam Jeelani, Dan Sato,
Tsuyoshi Karaki, Shigeharu Harada 15:30 - 15: 45 Unique
binding of chromodomains to unmethylated histone H3 tail in Entamoeba histolytica Ibne Karim M. Ali*, Kimberly R. Wiggins, Sepideh Khorasanizadeh and
William A. Petri, Jr 15:45 - 16:00 Identification
of a putative receptor of the major virulence factor cysteine protease in the enteric protist Entamoeba histolytica Kumiko Nakada-Tsukui*, Atsushi Furukawa, Yoko Yamada, Tomoyoshi
Nozaki 16:00 - 16:15 COFFEE BREAK Session
IV (16:15 - 17:30) Room Camino
Real Protein
Structure B Chairpersons: Sharon
Reed, Luis Brieba and Luis Felipe Padilla 16:15 - 16:30 Homology modeling reveals
differences between expressed cysteine proteinases of Entamoeba histolytica Chen He, Iain D. Kerr, Linda S. Brinen, Ken Hirata, James McKerrow,
William R. Roush, Sharon Reed* 16:30 - 16:45 Crystal
structure of native o-acetyl-serine sulfhydrylase from Entamoeba histolytica and its complex with natural compounds: leads to inhibitor development Isha Raj, Manish Kumar, Shivesh Kumar, S. Gourinath* 16:45 - 17:00 Role of the
preproregion in inhibition and folding of the cysteine proteinase EhCP112 of Entamoeba histolytica Olga
Zamudio-Prieto*, L. Iztel Quintas-Granados, Jaime Ortega-Lopéz 17:00 -17:30 Discussion 17:30-19:30 Poster Session I and Poster Discussion Scientific Program Session V (9:00 - 11:00) Room Camino Real Protein
Function Chairpersons: Nancy
Guillén and Alok Bhattacharya 9:00 - 9 :15 Cytoskeleton elements providing insight in the pathogenesis of Entamoeba histolytica Chung Chau Hon, Roman Thibeaux, Ruchi Jain,
Sabrina Marion, Samantha Blazquez,
Nancy Guillén* 9:15 - 9:30 Selective
activation of small GTPases (Rho, Rac, Cdc42) and actin cytoskeleton
structuration in Entamoeba histolytica
trophozoites during liver abscess development Verónica Ivonne Hernández-Ramírez*, Viola Leticia Pérez-Castillo,
Amelia Rios, Amelia Angel, Carlos Osorio, Blanca Reyes-Márquez, Patricia
Talamás-Rohana 9:30 - 9:45 Basic
characterization of a class of novel multiple EF-HAND calcium binding proteins
of Entamoeba histolytica Narendra Padhan, R. Jain, N. Sahoo, P. Chakrabarty, KVR. Chary, S.
Gourinath, S. Bhattacharya, A. Bhattacharya* 9:45 - 10:00 An
essential light chain of myosin II is part of the myosin IIA complex that regulates oriented migration to chemotactic
molecules Juan Daniel Díaz-Valencia*, Elizabeth Franco, Nicolás Villegas- Sepúlveda,
José Manuel Galván-Moroyoqui, Isaura Meza 10:00 - 10:15 Entamoeba
histolytica sirtuin-EhSir2a, interacts with multiple substrates and affects chromosome
segregation and cell division Somasri Pal*,
Anuradha Lohia 10:15 - 10:30 Thioredoxin reductase as activator and target
of metronidazole activity against Entamoeba histolytica and
Trichomonas vaginalis David Leitsch, Daniel Kolarich, Marina Binder,
Friedrich Altmann, Michael Duchene* 10:30-11:00 Discussion 11:00-11:30 COFFEE BREAK Session VI (11:30 - 13:30) Room Camino Real Intracellular
Traffic Chairpersons: Lesly
Temesvari and Isaura Meza 11:30 - 11:45
Signaling role
of Myeong Heon Shin*, Ju Young Kim, Young Ah
Lee, Kyeong Ah Kim 11:45 - 12:00
Alterations of endoplasmic reticulum morphology and
vesicle trafficking in cells expressing mutant EhRabA, a unique Rab GTPase of Entamoeba
histolytica Brenda H.
Welter, Lesly A. Temesvari* 12:00 - 12:15 Biochemical
characterization of Sarah Buss*, William A. Petri, Jr 12:15 - 12:30 Characterization
of the endocytic pathway of ferritin by Entamoeba
histolytica Luis Fernando López Soto*, Carolina Piña-Vázquez,
Nidia León-Sicairos, Arturo González-Robles, Mireya de 12:30 - 12:45 Proteomic analysis of actin stress
fibers formed after trophozoites-fibronectin (FN) interaction Verónica Ivonne
Hernández-Ramírez*, Edgar Rodrigo Guzman-Bautista, Febe Cáceres, Patricia
Talamás-Rohana 12:45 - 13:00 A novel phospholipase C of Entamoeba histolytica, a homologue of
neutral sphingomyelinases Claudia Leticia Mendoza-Macias*, Minerva Paola Barrios-Ceballos, Fernando
Anaya-Velázquez, Felipe Padilla-Vaca 13:00-13:30 Discussion 13:30 -15:00 LUNCH Session
VII (15:00 - 16:00) Room Camino
Real Encystment A Chairpersons: Graham
Clark and Fernando Anaya 15:00 - 15:15 A
developmentally regulated MYB domain protein regulates expression of
encystation-specific genes in Entamoeba
histolytica Gretchen M. Ehrenkaufer*, Jason A. Hackney, Upinder
Singh 15:15 - 15:30 In vitro encystment
of Entamoeba
histolytica by
oxidative stress and oligoelements Hugo Aguilar-Díaz*, Juan
P. Laclette, Julio C. Carrero 15:30 - 15:45 Identification of small
molecules that inhibit Entamoeba
excystation and encystation Biswa Nath Mitra*, Gabriele Pradel, Daniel Eichinger 15:45 - 16:00 Dynamics of Jaishree Paul*,
Nishant Singh, Sudha Bhattacharya 16:00 - 16:15 COFFEE BREAK Session VIII (16:15 - 17:30) Room Camino Real Encystment B Chairpersons: Daniel
Eichinger and Eva Avila 16:15 - 16:30 Lysozyme effect on the viability of Entamoeba
invadens immature cysts Luis Antonio Pérez García*, Eva E. ávila 16:30 - 16:45 Entamoeba histolytica MYB Elisa Azuara*,
Selene Zárate, Eric Meneses, Luis Brieba, Helios Cárdenas 16:45 - 17:00 Autophagy in Entamoeba
histolytica Karina
Picazarri*, Atsushi Furukawa, Dan Sato, Kumiko Nakada-Tsukui, Tomo Nozaki 17:00 - 17:30 Discussion 17:30-19:30
Poster Sessions II and Poster
Discussion Scientific Program Friday
November 27 Session IX (9:00 - 11:00) Room Camino Real Epidemiology Chairpersons: Jaco
Verweij and Cecilia Ximénez 9:00 - 9:15 Longitudinal study of molecular epidemiology of Entamoeba histolytica in a
big institution for psychiatric patients in Dar-Der Ji*,
Shin-Min Lee, Hung-Yin Teng, Yun-Hsien Chan, Shui-Yuan Hsu, Wei-Hung
Hsiao, Yeong-Sheng Lee, Jung-Sheng Chen 9:15 - 9:30 Amoebiasis in Leon, Nicaragua: over-diagnosis and
overtreatment Byron Leiva*,
Jadwiga Winiecka- Krushnel, Ewert Linder 9:30 - 9:45 Hidden diversity of Entamoeba moshkovskii in
humans C. Graham Clark*,
Bobbi S. Pritt, Jeffrey D. Silberman 9:45 - 10:00 Entamoeba histolytica and Entamoeba dispar infection patterns in
HIV+/AIDS patients: longitudinal follow up Patricia Morán*, Liliana Rojas, Alejandro
Gómez, Gabriela García, Fernando Ramos, Alicia Valadez, Enrique González,
AngÈlica Limón, Olivia Valenzuela, Cecilia Ximénez 10:00 -
10:15 Challenges in diagnosis of amoebic liver
abscess in a Hospital University: Malaysia experience Zeehaida, M, Lim BH*, Wan Nor
Amilah, WAW, Amry, AR, Hassan S., Sarimah A., Rahmah, N. 10:15 - 10:30 Two different chitinase genotypes in a patient with an amebic liver
abscess: a case report Cecilia Ximénez*, Olivia Valenzuela,
Patricia Morán, Fernando Ramos, Jorge I. Cardoza, Gabriela García, Alicia
Valadez, Liliana Rojas, Adriana Garibay, Enrique González 10:30 -11:00 Discussion 11:00 - 11:30 COFFEE
BREAK Session X (11:30 - 13:30) Room Camino Real Immunology Chairpersons: William
Petri Jr. and Mineko Shibayama 11:30 - 11:45 Innate immunity in hamsters and rats with
liver vagotomy and liver amoebiasis Martín Humberto Muñoz Ortega*, Javier Ventura Juárez, Andrés Quintanar
Stephano, Mario García Lorenzana 11:45 - 12:00 Entamoeba histolytica and enteropathogenic bacteria induce expression of Toll-receptor in
human intestinal cells José Manuel Galván-Moroyoqui*, M. del Carmen Domínguez-Robles, Isaura Meza 12:00 - 12:15 The cysteine protease EhCP-a5 is essential for Entamoeba
histolytica invasion and for inducing inflammatory responses in
an ex-vivo human colon model Elizabeth
LabruyËre*, D. Bansal, P. Roux, P. Ave, S. Kerneis,
A.S. Leguern, P. Frileux, A.C. Baglin, A. Danckaert, M.C. Prevost , R. Bracha,
D. Mirelman, N. Guillén 12:15 - 12:30 Natural killer T cells activated by the Entamoeba histolytica
lipopeptidophosphoglycan are critically important to control amoebic liver
abscess Hannelore Lotter, Nestor González-Roldán,
Artur J. Ulmer, Otto Holst, Thomas Jacobs, Egbert Tannich* 12:30 - 12:45 Lipopeptidophosphoglycan, a glycolipid
from Entamoeba histolytica that
functions as antigen and adjuvant Nestor
Gonzalez-Roldan*, Hannelore Lotter Buko Lindner, Arthur Ulmer, Thomas Jacobs,
Otto Holst, Egbert Tannich 12:45 - 13:00 Protection against intestinal amoebiasis by recombinant
vaccine is mediated by T cell and dependent on IFN-γ Xiaoti Guo*,
Eric Houpt, William A. Petri Jr 13:00 -13:30 Discussion 13:30 - 15:00 LUNCH Session XI
(15:00 - 15:45) Room Camino Real Experimental Amebiasis A Chairpersons: Egbert
Tannich and Ruy Pérez Tamayo 15:00 - 15:15 Response of Entamoeba
histolytica to oxidative and nitrosative stress: implications for amoebic
pathogenesis João B. Vicente*,
Gretchen Ehrenkaufer, Lígia M. Saraiva, Miguel Teixeira, Upinder Singh 15:15 - 15:30 Comparison
of two syngenic Entamoeba histolytica
cell lines with different pathogenic phenotypes Iris Bruchhaus*, Laura
Biller, Paul H. Davis, Samuel L. Stanley Jr., Ottmar Janssen, Hendrik Schmidt,
Eberhard Krause, Christoph Gelhaus, Hannelore Lotter, Egbert Tannich 15:30 - 15.45 Role of transforming growth factor b (TGF-b) during
the regeneration of amoebic liver abscess Mineko
Shibayama*, Jesús Serrano-Luna, Jessica Velázquez, Víctor Tsutsumi Session
XII (16:00 - 17:30) Room
Camino Real Experimental Amebiasis B Chairpersons: Adolfo
Martínez-Palomo and Jonathan Ravdin 16:00 - 16:15 Development
of an effective amebiasis subunit mucosal vaccine in a novel primate model of Entamoeba histolytica intestinal
infection and invasive colitis Jonathan I.
Ravdin*, Mohamed D. Abd Alla, Roman Wolf, Gary L. White, Jaco J.
Verweij, Mie-Jie Zhang 16:15 - 16:30 Prostaglandin E2
produced by Entamoeba histolytica alters human colonic epithelial
barrier function Manigandan Lejeune*, and Kris Chadee 16:30 - 16:45 Comparison of Entamoeba histolytica and Entamoeba
dispar infection in human intestine Samuel L.
Stanley Jr*, Sarah A. Evans 16:45 - 17:15 Discussion 17:30-19:00 Poster session